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Coherent Raman Scattering Microscopy

Series in Cellular and Clinical Imaging

Format: Paperback / softback
Publisher: Taylor & Francis Ltd, London, United Kingdom
Imprint: CRC Press
Published: 16th Apr 2018
Dimensions: w 246mm h 172mm d 30mm
Weight: 1640g
ISBN-10: 1138199524
ISBN-13: 9781138199521
Barcode No: 9781138199521
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Synopsis
The First Book on CRS Microscopy Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Scattering Microscopy explains how CRS can be used to obtain a point-by-point chemical map of live cells and tissues. In color throughout, the book starts by establishing the foundation of CRS microscopy. It discusses the principles of nonlinear optical spectroscopy, particularly coherent Raman spectroscopy, and presents the theories of contrast mechanisms pertinent to CRS microscopy. The text then provides important technical aspects of CRS microscopy, including microscope construction, detection schemes, and data analyses. It concludes with a survey of applications that demonstrate how CRS microscopy has become a valuable tool in biomedicine. Due to its label-free, noninvasive examinations of living cells and organisms, CRS microscopy has opened up exciting prospects in biology and medicine-from the mapping of 3D distributions of small drug molecules to identifying tumors in tissues. An in-depth exploration of the theories, technology, and applications, this book shows how CRS microscopy has impacted human health and will deepen our understanding of life processes in the future.

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"This book makes a seminal contribution to the field of biomedical imaging, and provides the reader with an excellent introduction and in-depth descriptions of the latest and greatest implementations of coherent Raman imaging technology-enough to jump start a new program or infuse new life into an existing one. The clear descriptions of a wide range of applications show how the methods are being used at present and indicate many different and varied future paths. This book is an excellent addition to the bookshelves of biomedical imaging practitioners and researchers in related fields, including surface analysis, process control, forensics, and others."
-David S. Moore, Los Alamos National Laboratory, Analytical and Bioanalytical Chemistry, 2013

"Interested readers can find an excellent mix of [optical platforms and applications given a solid theoretical approach] in this book, which is the first one dealing in a comprehensive and exhaustive way with CRS microscopy and related techniques. No one involved in optical microscopy and spectroscopy should miss this text, which is able to offer new exciting scenarios and, for sure, at least one new idea after reading."
-Alberto Diaspro, Italian Institute of Technology (IIT), Journal of Biomedical Optics, 2014