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Synthetic Protein Switches

Methods and Protocols. Methods in Molecular Biology 1596

Edited by Viktor Stein
Genres: Biotechnology
Format: Hardback
Publisher: Humana Press Inc., Totowa, NJ, United States
Published: 15th Mar 2017
Dimensions: w 178mm h 254mm d 21mm
Weight: 834g
ISBN-10: 1493969382
ISBN-13: 9781493969388
Barcode No: 9781493969388
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Synopsis
This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, -lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.

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