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Fibrosis

Methods and Protocols. Methods in Molecular Biology 1627

Edited by Laure Rittie
Format: Hardback
Publisher: Humana Press Inc., Totowa, NJ, United States
Published: 25th Aug 2017
Dimensions: w 178mm h 254mm d 30mm
Weight: 1161g
ISBN-10: 1493971123
ISBN-13: 9781493971121
Barcode No: 9781493971121
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Synopsis
This volume describes state-of-the-art protocols that serve as "recipes" for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis in the lungs, skin, liver and heart, and generating transgenic mouse models. Part II discusses cell culture systems, where the chapters explore cell types important for the development of fibrosis. Part III looks at the purification, quantification, and analysis of the ECM proteins, and Part IV describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy (AFM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Fibrosis: Methods and Protocols is a valuable resource aimed at outstanding quality and repeatability of research experiments in the fibrosis field.

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