The extent of electrophoretic partition techniques has expanded significantly since Tiselius' pioneer work for which he got the Nobel Prize. Improvement of these techniques has advanced from paper, cellulose acetate membranes and starch gel electrophoresis to molecular sifter, disk, SDS, and immunoelectrophoresis lastly to isoelectric focusing yet in addition to high resolution two-dimensional electrophoresis. Together with silver and gold staining, autoradiography, fluorography and blotting, these strategies bear better determination, sensitivity and specificity for the investigation of proteins. And gel electrophoresis has turned out to be a one of a kind strategy for DNA sequencing while high resolution two-dimensional electrophoresis has smoothed the captivating way from separation of the protein to the gene through amino acid sequencing and after gene cloning, to protein making. The range of expository conceivable outcomes have turned out to be varied to the point that a discussion of electrophoretic partitioning techniques appears to be alluring for newbies as well as for experienced professionals. The purpose of this book is to give a depiction of present day Electrophoresis practices with novel methodologies and controls which are extensively playing a pivotal role in the separation techniques development and modernization. This book introduces a snapshot of the all-around prospecting Electrophoresis world alongside the most recent technological issues and how they are being tended to. This book is of interest for graduate level audience who are keen to get in touch with Electrophoretic systems, methodologies and technologies. This book is also of interest for researchers and technical staff working in today's innovative separation processes based world. The first chapter offers a general sight of Electrophoresis, methodologies, technologies and techniques which are used for controlling them. In the later chapters Isotachophoresis, Isoelectric focusing, Blotting, Interpretation of electropherograms, Proteome Analysis, Equipment and Instrumentation, Titration curve analysis and Various inoovative electrophoresis based Methods have been explained.