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Germ Cell Protocols

Molecular Embryo Analysis,Live Imaging,Transgenesis,and Cloning. v.2

By (author) Helde Schatten
Edited by Helde Schatten
Format: Online resource
Publisher: Humana Press Inc., Totowa, NJ, United States
Published: 29th Feb 2004
ISBN-10: 1592597416
ISBN-13: 9781592597413
Barcode No: 9781592597413
Synopsis
For volume 2 alone: A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.

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