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Germ Cell Protocols

Sperm and Oocyte Analysis. v.1

By (author) Helde Schatten
Edited by Helde Schatten
Format: Online resource
Publisher: Humana Press Inc., Totowa, NJ, United States
Published: 29th Feb 2004
ISBN-10: 1592597440
ISBN-13: 9781592597444
Barcode No: 9781592597444
Synopsis
A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of stallion spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. For both volumes:

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