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mRNA Processing and Metabolism

Methods and Protocols

Format: Online resource
Publisher: Humana Press Inc., Totowa, NJ, United States
Published: 31st Jan 2004
ISBN-10: 1592597505
ISBN-13: 9781592597505
Barcode No: 9781592597505
Synopsis
A collection of readily reproducible techniques for the study of mRNA processing and metabolism. These cutting-edge techniques range from cotranscriptional processing events that occur while the mNA is engaged with elongating RNA polymerase II, to in vivo and in vitro splicing and its biochemical analysis, and alternative splicing. Additional methods cover mRNA export, the recovery and analysis of mRNP complexes, cytoplasmic translation, mRNA degradation in vivo and in vitro, and the controversial concept of nuclear translation. Each proven protocol is described in step-by-step detail and contains a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding know pitfalls.

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