This Safety Guide provides guidance on the development, content and use of operational limits and conditions (limits on plant operating parameters) and on the operating procedures that affect them. It recommends how to meet the requirements established in Safety Standards Series No. NS-R-2, Safety of Nuclear Power Plants: Operation (2000), setting out the responsibilities of the operating organization in setting, modifying and documenting operational limits and conditions and ensuring compliance with them. It supersedes Safety Series No. 50-SG-O3, Operational Limits and Conditions for Nuclear Power Plants (1979).