This presents an in-depth look at recombinant DNA methodology, geared for senior undergraduates and graduate students majoring in the biosciences. Basic techniques and alternative methods in recombinant DNA methodology are discussed. The book focuses on the application of techniques, including introductory and background material for each technique, and provides the "how" and "why" of the manipulations to give training and understanding in the field. It presents tested, step-by-step methods for different techniques and experiments, and covers site-directed mutagenesis and yeast cloning. Tips on trouble-shooting are also provided.